Journal: eLife
Article Title: Structural intermediates observed only in intact Escherichia coli indicate a mechanism for TonB-dependent transport
doi: 10.7554/eLife.68548
Figure Lengend Snippet: Shown in ( A ) is a side view of sites 90 and 237 (PDB ID:1NQH). In ( B ) is shown the distribution for the V90R1-S237R1 spin pair in the absence and presence of R14A with and without vitamin B 12 , where the histogram represents prediction from the vitamin B 12 bound structure (PDB ID: 1NHQ). A significant portion of the predicted distance distribution is below the minimum range observable using double electron-electron resonance (DEER) (1.5–2 nm). Shown in ( C ) are the raw data and background for the V90R1-S237R1 spin pair without and with the R14A mutation, and in ( D ) are the background correct DEER data. These data were analyzed using the DeerNet routine in DEERAnalysis . Histograms are predicted distances generated from the in surfo crystal structures (PDB ID: 1NQG (blue) and PDB ID: 1NQH (red)) using MMM .
Article Snippet: Software, algorithm , DeerAnalysis (v. 2019) , Gunnar Jeschke (ETH Zürich) , MATLAB routine for the analysis of pulse EPR data , Used to examine DEER data.
Techniques: Mutagenesis, Generated